Tips for Working with Magnetic Beads
Purification is a common procedure in most molecular biology experiments, such as the removal of short primers, unincorporated dNTPs, enzymes, and salts from PCR reactions and other impurities. There are various types of purification methods, including filtration, centrifugation and separation. Separation has become a very popular method in life sciences and various types of solid phase support are used. This includes agarose beads, sepharose beads, silica beads, and magnetic beads. Separation using magnetic beads is the quickest, cleanest and most efficient technique out of all the bead separation methods.