Cell Separation and Detection

MagVigen™ for Cell Separation and Detection

MyQuVigen™ for Imaging, Cell Separation and Detection

Citations:

1. Asymmetric division through a reduction of microtubule centering forces
2. Inflammatory responses to acute elevations of carbon dioxide in mice.
3. High-throughput full-length single-cell mRNA-seq of rare cells.
4. AFP mRNA level in enriched circulating tumor cells from hepatocellular carcinoma patient blood samples is a pivotal predictive marker for metastasis.
5. Y-box protein 1 is required to sort microRNAs into exosomes in cells and in a cell-free reaction

 MagVigen™ for Cell Separation & Detection

Figure 1. SPRI beads based cell separation workflow.

1. Incubate: Incubate cells with MagVigen™ nanoparticles to bind the targeted cells to nanoparticles.
2. Ioslate: The beads respond to a magnetic force (by use of a magnet, e.g. magnetic separation rack), allowing bound material to be rapidly and efficiently separated from the rest of the sample. Remove unbound materials by aspiration.
3. Wash: Wash the nanoparticle-bound targets using the magnet. Release the bound target cells from the nanoparticle.

MagVigen magnetic nanoparticles can be used to isolate circulating tumor cells (CTC). With a simple procedure and fewer washing steps, cell separation can be done in a shorter time.

With the conjugation to anti-CD3 & CD28 antibobdy, MagVigen nanoparticles are ideal to be used in CAR technology to activate human T cells. They act as antigen-presenting cells and activate resting T cells from peripheral blood mononuclear cells (PBMCs) as well as purified T cells.

Figure 2. H1650 cell capture using Streptavidin (SA) nanoparticles from different vendors. NVIGEN MagVigen beads show much higher CTC capture yield compared to other products on market.

Figure 3. Human T cell expansion using NVIGEN’s anti-CD3 & CD28 nanoparticles. Up to 577-fold expansion is achieved in 11 days.

 MyQuVigen™ for Imaging, Cell Separation and Detection

MyQuVigen™ – A unique combination of fluorescent and magnetic nanoparticles providing high magnetic moment and bright stable fluorescence, ideal for controllable magnetic manipulation with extensive, multiplexed fluorescence imaging.

MyQuVigen™ fluorescent magnetic nanoparticles can be conjugated with streptavidin, primary or secondary antibodies for magnetic cell separation and fluorescence based cell identification application.

Figure 4. MyQuVigen™ fluorescent magnetic nanoparticles with bright and stable fluorescence property. (A) MyQuVigen™ fluorescent magnetic nanoparticles with 3 different emission color, red(615 nm), yellow (585 nm) and green (535 nm). Single nanoparticle fluorescence could be clearly imaged. (B) Fluorescence of MyQuVigen™ fluorescent magnetic nanoparticles are very stable allowing long term tracking and imaging after 12 days of nanoparticle uptake in Hela cells.

The concurrent magnetic cell separation and fluorescence imaging enabled by MyQuVigen™ fluorescent magnetic nanoparticles omit the additional cell staining steps from traditional magnetic cell separation approach, which speeds up experimental flow and improves cell viability. Cells magnetically isolated using MyQuVigen™ fluorescent magnetic nanoparticles are tagged with strong fluorescent signals and can be directly imaged using fluorescence microscopes or analyzed with other fluorescence based cell analysis methodology, such as FACS, fluorescence assisted cell sorting or other single cell analysis.

Figure 5. Two different types of circulating tumor cells were isolated with MyQuVigen™ fluorescent magnetic nanoparticles from a whole blood sample. Tumor cell surface markers were stained with these fluorescent magnetic nanoparticles by binding with antibodies conjugated to nanoparticle surfaces.

MyQuVigen™ fluorescent magnetic nanoparticles have optimal surface chemistry hence they feature high surface binding specificity. They could be utilized to effectively isolate low abundance cells from a mixture of cell populations from tissue, organ or whole blood samples. Cancer cells, immune cells or stem cells could all be specifically isolated and identified with MyQuVigen™ fluorescent magnetic nanoparticles.

Advantages:

• Strong magnetic property
• Bright stable fluorescence signal
• Smaller nanoparticle size with improved binding specificity
• Omits additional fluorescence labeling to speed up experimental flow
• Ideal for cell separation and magnetic manipulation

Applications:

• Multiplexed Fluorescence Imaging
• Magnetic Manipulation
• FACS
• DNA, RNA, protein sample prep and detection
• Cell separation and detection

Learn which scientists are citing this product series on what projects.