Cell Separation and Detection

 MagVigen™ for Cell Separation & Detection

MagVigen magnetic nanoparticles can be used to isolate circulating tumor cells (CTC). With a simple procedure and fewer washing steps, cell separation can be done in a shorter time.

With the conjugation to anti-CD3 & CD28 antibobdy, MagVigen nanoparticles are ideal to be used in CAR technology to activate human T cells. They act as antigen-presenting cells and activate resting T cells from peripheral blood mononuclear cells (PBMCs) as well as purified T cells.

Figure 1. H1650 cell capture using Streptavidin (SA) nanoparticles from different vendors. NVIGEN MagVigen beads show much higher CTC capture yield compared to other products on market.

Figure 2. Human T cell expansion using NVIGEN’s anti-CD3 & CD28 nanoparticles. Up to 577-fold expansion is achieved in 11 days.

 MyQuVigen™ for Imaging, Cell Separation and Detection

MyQuVigen™ – A unique combination of fluorescent and magnetic nanoparticles providing high magnetic moment and bright stable fluorescence, ideal for controllable magnetic manipulation with extensive, multiplexed fluorescence imaging.

MyQuVigen™ fluorescent magnetic nanoparticles can be conjugated with streptavidin, primary or secondary antibodies for magnetic cell separation and fluorescence based cell identification application.

Figure 3. MyQuVigen™ fluorescent magnetic nanoparticles with bright and stable fluorescence property. (A) MyQuVigen™ fluorescent magnetic nanoparticles with 3 different emission color, red(615 nm), yellow (585 nm) and green (535 nm). Single nanoparticle fluorescence could be clearly imaged. (B) Fluorescence of MyQuVigen™ fluorescent magnetic nanoparticles are very stable allowing long term tracking and imaging after 12 days of nanoparticle uptake in Hela cells.

The concurrent magnetic cell separation and fluorescence imaging enabled by MyQuVigen™ fluorescent magnetic nanoparticles omit the additional cell staining steps from traditional magnetic cell separation approach, which speeds up experimental flow and improves cell viability. Cells magnetically isolated using MyQuVigen™ fluorescent magnetic nanoparticles are tagged with strong fluorescent signals and can be directly imaged using fluorescence microscopes or analyzed with other fluorescence based cell analysis methodology, such as FACS, fluorescence assisted cell sorting or other single cell analysis.

Figure 4. Two different types of circulating tumor cells were isolated with MyQuVigen™ fluorescent magnetic nanoparticles from a whole blood sample. Tumor cell surface markers were stained with these fluorescent magnetic nanoparticles by binding with antibodies conjugated to nanoparticle surfaces.

MyQuVigen™ fluorescent magnetic nanoparticles have optimal surface chemistry hence they feature high surface binding specificity. They could be utilized to effectively isolate low abundance cells from a mixture of cell populations from tissue, organ or whole blood samples. Cancer cells, immune cells or stem cells could all be specifically isolated and identified with MyQuVigen™ fluorescent magnetic nanoparticles.

Advantages:

• Strong magnetic property
• Bright stable fluorescence signal
• Smaller nanoparticle size with improved binding specificity
• Omits additional fluorescence labeling to speed up experimental flow
• Ideal for cell separation and magnetic manipulation

Applications:

• Multiplexed Fluorescence Imaging
• Magnetic Manipulation
• FACS
• DNA, RNA, protein sample prep and detection
• Cell separation and detection

 

MagVigen™ for Cell Separation and Detection

Name	Cat#	Price (1ml, USD)	Add to cart
MagVigen™ – Streptavidin, cell separation Product Sheet	21005C	1 ml $211.0010 ml $1,688.80100 ml $9,480.80
MagVgien-Streptavidin kit, cell separation Product Sheet	K21005C	1 ml $240.0010 ml $1,928.80100 ml $9,998.80
MagVigen™ – anti EpCAM, human Product Sheet	51001	$329
MagVigen™ – anti EpCAM, human Kit Product Sheet	K51001	$360
MagVigen™ – anti CD45, human Product Sheet	51002	$299
MagVigen™ – anti CD45, human Kit Product Sheet	K51002	$330
MagVigen™ – anti CD4, human Product Sheet	51003	$299
MagVigen™ – anti CD4, human Kit Product Sheet	K51003	$330
MagVigen™ – anti CD8a, human Product Sheet	51004	$299
MagVigen™ – anti CD8a, human Kit Product Sheet	K51004	$330
MagVigen™ – anti CD19, human Product Sheet	51005	$299
MagVigen™ – anti CD19, human Kit Product Sheet	K51005	$330
MagVigen™ – anti CD20, human Product Sheet	51006	$299
MagVigen™ – anti CD20, human Kit Product Sheet	K51006	$330

MyQuVigen™ for Imaging, Cell Separation and Detection

Name	Cat#	Price (1ml, USD)	Add to cart
MyQuVigen™ – SH surface, 535, 585, 615 or 635 nm,emission Product Sheet	41001	$361
MyQuVigen™ – NH2 surface, 535, 585, 615 or 635 nm emission Product Sheet	41002	$361
MyQuVigen™ – protein A conjugates, 535, 585, 615 or 635 nm emission Product Sheet	41003	$411
MyQuVigen™ – protein G conjugates, 535, 585, 615 or 635 nm emission Product Sheet	41004	$424
MyQuVigen™ – streptavidin conjugates, 535, 585, 615 or 635 nm emission Product Sheet	41005	$424
MyQuVigen™ – anti rabbit IgG conjugates, 535, 585, 615 or 635 nm emission Product Sheet	41006	$448
MyQuVigen™ – anti mouse IgG conjugates, 535, 585, 615 or 635 nm emission Product Sheet	41007	$448
MyQuVigen™ – customize	41008	Contact Us