| 产品名称 | 应用 | 目录号 | 规格/价格(1ml, USD) | 加入购物车 |
| MagVigen™ – Plasma DNA Capture Kit | 对血浆中的 cfDNA 的捕获 Product Sheet |
K61003- |
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产品介绍: MagVigen™ Plasma DNA Capture 磁性纳米颗粒专为捕获血液中的cfDNA而设计，为科研， 分子检测工作者提供了一种简单、快速、高效的血液cfDNA提取方法。整个实验过程中无需离心或者过柱。DNA提取可以线性比例扩大，输入血浆范围为100 uL至10mL 。分离出的cfDNA 可以用Agilent Bioanalyzer 直接定量分析。
下游应用: PCR, DNA测序，二代测序技术(NGS), 以及测序文库构建。
产品特点:
- 高产量
- 高质量DNA提取
- 工作流程简单
- 血浆体积可线性扩展
- 质量稳定，提取结果高度一致
磁性工作流程
图 1. 磁性工作流程.
MagVigen™ cfDNA提取的工作流程可以从 100 µl 线性扩展到 10 ml 的血浆输入样品，为下游PCR或测序分析提供了高质量的 cfDNA，提取的cfDNA 的产量和质量明显优于市场上的其他方法。
MagVigen™ 能实现简单高效的 DNA 提取，是自动化的理想方案。
图 2. NVIGEN cfDNA 试剂盒为下游 PCR 和 NGS library 提供更高的产量和更高的质量的 DNA。
图 3. 生物分析仪谱显示 NVIGEN 试剂盒比 QIAGEN-ccf 试剂盒提取的 cfDNA 获得更高的产量和更高品质的NGS library。
图 4. 4 ml血浆输入，NVIGEN 试剂盒比 QIAGEN-ccf 试剂盒提取的 cfDNA 产量更高。
图 5. DNA 回收效率。原始 ladder DNA（红线）和回收的 DNA（蓝线）的生物分析仪文件。
图 6. cfDNA extraction yield and size from 1.5 ml plasma samples with (blue) and without (red) spiking in DNA. Calculated yield of spiking in DNA by subtraction of endogenous cfDNA from total DNA were 80% using picogreen assay and 91% with bioanalyzer quantification.
图 7. Higher capture rate for smaller sizes of cfDNA is possible with NVIGEN MagVigen-cfDNA extraction kit.
图 8. The yield of cfDNA increases in proporation to the volume of plasma input. Captured cfDNA was quantified by pico-green analysis.
图 9. cfDNA extraction yield was quantified by pico-green analysis following DNA isolation with the MagVigen™ Plasma DNA Capture Kit and kit C.
图 10. Beta-globin level and GAPDH levels were estimated by PCR analysis.
图 11. NIPT Sequencing library constructed with NVIGEN cfDNA and DNA sizing kits in genomic diagnostic company using real clinical samples.
图 12. Much better differentiation between patient plasma and normal plasma was achieved using MagVigen cfDNA extraction followed by sequence specific target capture with MagVigen-Streptavidin nanobeads vs. MyOne-Streptavidin beads. The qPCR Ct differences between patient and normal plasma samples were over 9 when using MagVigen-Streptavidin Nanobeads (Cat# K61002) and less than 2 when using MyOne-Streptavidin beads, corresponding to over 100x better differentiation power when referring to ctDNA copy numbers of the original plasma samples of 2^9 vs. 2^2.