MagVigen™ Plasma DNA Capture Kit Cat# K61003
MagVigen™ nucleic acids isolation solution can be scaled up linearly from 100 µl of plasma to 5ml of plasma. cfDNA isolated with MagVigen™ can be used directly for PCR or sequencing library construction. The yield and quality of cfDNA is comparable with column-based protocols.
And MagVigen™ allows simple and efficient DNA extraction ideal for automation.
Figure 1. The left figure above shows DNA extraction results of 50 ng DNA (82 bp) spiked into 600 µl of plasma, using two different batches of beads and for multiple extraction experiments. Aliquots of DNA elution were used for amplification. An average yield of 50% could be achieved for 82bp DNA.
Figure 2. 500 ng Ultra Low DNA Ladder were spiked into 120 µl of freeze-thawed human plasma. DNA extraction from the plasma sample was achieved using MagVigen™ – Plasma DNA Capture Kit (Cat# K61003). DNA recovery rate were quantified by PicoGreen® and recovered Ultra Low Ladder were analyzed using gel-electrophoresis. DNA with sizes as low as 50bp could be captured.
Figure 3. Efficiency of DNA recovery. Bioanalyzer files of original ladder DNA (red line) and recovered spiked-in ladder DNA after extraction (blue line).
Figure 4. cfDNA extraction yield and size from 1.5 ml plasma samples with (blue) and without (red) spiking in DNA. Calculated yield of spiking in DNA by subtraction of endogenous cfDNA from total DNA were 80% using picogreen assay and 91% with bioanalyzer quantification.
Figure 5. Higher capture rate for smaller sizes of cfDNA is possible with NVIGEN MagVigen-cfDNA extraction kit.
Figure 6. cfDNA extracted from 100 µl to 5 ml of plasma was quantified by pico-green analysis.
Figure 7. cfDNA extraction yield was quantified by pico-green analysis following DNA isolation with the MagVigen™ Plasma DNA Capture Kit and kit C.
Figure 8. Beta-globin level and GAPDH levels were estimated by PCR analysis.
Figure 9. NIPT Sequencing library constructed with NVIGEN cfDNA and DNA sizing kits in genomic diagnostic company using real clinical samples.
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|MagVigen™ – Plasma DNA Capture Kit|| For DNA capture from plasma samples. Proteinase K and buffer included.