Developing Serological On-Bead-ELISA to Detect COVID-19 Virus SARS-Cov-2

Highly sensitive, simple and rapid workflow.

nvigen-sars-cov-2-testing-on-bead-elisa-workflow-illustration

Figure 1. NVIGEN SARS-Cov-2 testing On-Bead-ELISA workflow illustration.

  1. Magnetic beads coated with capture ligand, antigen or antibody, blocking with BSA. 1 Magnetic rack can process 16 of 1.5 ml beads solution, enough for 2400 to 4800 reactions.

  2. 1 step incubation 20 min.

  3. Magnetic Separation and wash 3x easily implemented with 96-well magnetic plate 15 min.

  4. Add chromogenic substrate or enhanced chemiluminescent solution for signal readout 5 min.

nvigen-on-bead-elisa-immunodiagnostics-detection-example

Figure 2. NVIGEN On-Bead-ELISA immunodiagnostics example experiment details. Similar testing workflow can be developed for SARS-Cov-2 detection with capture ligands conjugated to NVIGEN magnetic nanobeads surfaces to capture serological antibodies such as IgG and IgM and other immune responses against SARS-CoV-2 infection. Figure shows NVIGEN beads-anti Mouse IgG antibody conjugates (10ul, 3ul) vs. Control NVIGEN beads w/o anti Mouse IgG Ab.

nvigen-on-bead-elisa-readout-plot

Figure 3. NVIGEN On-Bead-ELISA Immunodiagnostics example readout plot. The experiment condition is outlined in Figure 2. The results from 10 ul of NVIGEN beads-capture antibody conjugates experiment set are plotted. This experiment used one step, 20 min incubation of mixtures of 10 ul of NVIGEN beads- capture antibody conjugates, detection sample (mouse IgG), 25 ul of 1:1000 dilution of anti Mouse-IgG-HRP and 50 ul of 1% of BSA.

Mouse IgG 15 ng 1.5 ng 150 pg 15 pg
NVIGEN-anti Mouse IgG, 10 ul 1.1 0.7 0.3 0.17
NVIGEN-anti Mouse IgG, 3 ul 0.46 0.36 0.19 0.14
Control NVIGEN beads w/o anti Mouse IgG 0.05 0.05 0.049 0.049

Table 1. O.D. Reading of the Original Data of Figure 2 and 3 experiments.

NVIGEN products that can be applied for SARS-CoV-2 testing assay development:

On-Bead-ELISA Immunodiagnostics

  • 21005P, MagVigenTM-Streptavidin Beads
  • 51009, MagVigenTM-anti His-Tag
  • A20006, 16-tube Magnetic Separation Rack
  • A20008, 96-Well Magnetic Plate
  • 51200, MagVigenTM-Customized Conjugation of Proteins, Binding Ligands, Antibodies onto Bead Surface

Molecular Diagnostics Using qPCR or NGS

  • K61001, MagVigenTM-Easy DNA Select for NGS Library Prep or PCR Clean up
  • K61002, MagVigenTM-Streptavidin for Highly Sensitive Nucleic Acid Capture
  • 61006, MagVigenTM-DNA Probe, Customized Preparation of Oligo Conjugates