DNA Sizing for NGS DNA Library Purification

 MagVigen™ DNA Size Selection Kits

MagVigen™ – DNA Size Selection Kits allows simple and rapid isolation of DNA from a variety of crude sample materials (eg. blood sample, cell lysates, clinical specimens, and tissue samples). It enables efficient recovery of DNA and efficient removal of unwanted contaminants (ex. primers, unincorporated dNTPs, and salts)

MagVigen™ DNA Size Selection magnetic nanoparticles and kits have been optimized to capture DNA with desired cutoff sizes: >50bp, >100bp, >150bp, >200bp, >300bp, >400bp, >500bp, >700bp.

Figure 1. MagVigen™ DNA Size Selection Capture DNA Fragments of Desired Size. A DNA ladder input was successfully captured for >500bp, >400bp, >300bp, >200bp.

Figure 2. shows the capture efficiency of the MagVigen™ beads for DNA fragments at a range of sizes in three different buffer conditions. A DNA ladder input was successfully captured for >150bp, >75bp, >35bp.

Figure 3. Non-hybridization based DNA capture with MagVigen™ beads can be used in a wide range of temperature conditions.

Figure 4. A use case. Customer requires 200bp cut-off and the same protocol as Company A XP beads. We can see MagVigen™ as an XP alternative: 1. At same ratio, with similar yield, MagVigen™ achieved higher fidelity. XP captured a lot 100bp. 2. From Ratio 0.8, we can see the backward trend (0.8 up) MagVigen™ has much higher sensitivity.

MagVigen™ DNA Size Range Selection Kits

MagVigen™ – DNA Size Selection Kits enable you to fine tune your buffer condition for DNA size range selection.

Cat# Kr61001-300/600, Kf61001-500 focus MagVigen™ provides fast and cost-effective solution to isolating DNA of desired sizes for high-quality library construction and Next-Gen Sequencing (NGS).

Figure 5. Left shows salmon genomic DNA was separated based on 2 different size range selections. 300bp<DNA<600bp can be isolated for NGS. Right shows a 500bp focused band was isolated from DNA ladder input using similar approach.

In Summary, MagVigen™ nanoparticles features:

• Volume Ratio or Streptavidin Based DNA Selection
• Long bp DNA Selection Available
• Precise Size Range Selection
• High Yield
• Easy Workflow
• Fast Magnetic Response

Learn more about the basics & development of DNA Size Selection and DNA cleanup.

 

Name	Application	Cat#	Price (1ml, USD)	Add to cart
MagVigen™ – Easy DNA Size Selection Kit	Magnetic nanobeas for ratio based DNA size 100~500bp. Perfect XP alternative (same features & protocol). Product Sheet	K61001-Easy	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >700bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >700bp Capture Buffer, Elution Buffer Product Sheet	K61001-700	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >500bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >500bp Capture Buffer, Elution Buffer Product Sheet	K61001-500	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >400bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >400bp Capture Buffer, Elution Buffer Product Sheet	K61001-400	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >300bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >300bp Capture Buffer, Elution Buffer Product Sheet	K61001-300	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >200bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >200bp Capture Buffer, Elution Buffer Product Sheet	K61001-200	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >150bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >150bp Capture Buffer, Elution Buffer Product Sheet	K61001-150	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >100bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >100bp Capture Buffer, Elution Buffer Product Sheet	K61001-100	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >50bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >50bp Capture Buffer, Elution Buffer Product Sheet	K61001-50	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – 500bp Focus, DNA Selection Kit	Including MagVigen™ magnetic nanobeads, DNA fragment size selection of 500bp Capture Buffers, Elution Buffer Product Sheet	Kf61001-500 Focus	$339
MagVigen™ – 300/600 bp Range, DNA Select Kit	Including MagVigen™ magnetic nanobeads, DNA fragment size selection between 300 and 600 bp Capture Buffers, Elution Buffer Product Sheet	Kr61001-300/600	$339

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