DNA Sizing and Cleanup for NGS DNA Library Purification

Improve your DNA cleanup and size selection for high efficiency sequencing & diagnostics.

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Learn more about DNA clean-up what, why and how.

Product Features:

• Precise Size Range Selection
• High Yield High Cleanness
• Long bp DNA Selection Available
• Easy Magnetic Workflow
• Reliable and Consistent

Citations:

1. Mutant Huntingtin Affects Diabetes and Alzheimer’s Markers in Human and Cell Models of Huntington’s Disease.
2. Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancers
3. Discovery of Active Cis-Regulatory Elements and Transcription Factor Footprints in Nematodes Using Functional Genomics Approaches

MagVigen™ DNA Cleanup & Size Selection Kits

MagVigen™ – DNA Cleanup & Size Selection Kits allow simple and rapid isolation of DNA from a variety of crude sample materials (eg. NGS library, PCR constructs). It enables efficient recovery of DNA and efficient removal of unwanted contaminants (ex. primers, unincorporated dNTPs, and salts)

DNA library clean-up examples using Cat# k61001-Easy: MagVigen™ Easy DNA Cleanup & Size Selection Kit.

Before DNA Clean-up	After DNA Clean-up

Figure 1. MagVigen™ Easy DNA Cleanup & Size Selection Kit (K61001-Easy) outperforms conventional brand products for strongly contanminated NGS DNA Library sample (adapter dimer ratio 224%). After clean-up, adapter dimer over library is less than 3% using MagVigen™ (passes quality control (QC)) vs. more than 6% using AmPure XP (fails QC).

Before DNA Clean-up	After NVIGEN K61001-Easy DNA Clean-up
Tested by Agilent 2100 12000 Assay	Tested by Agilent 2100 High Sensitivity Assay

Figure 2. MagVigen™ Easy DNA Cleanup & Size Selection Kit (K61001-Easy) outperforms conventional brand products for significantly contanminated NGS DNA Library sample (adapter dimer ratio 186%). After clean-up, adapter dimer over library reduced to 2.3%.

Before DNA Clean-up	After DNA Clean-up

Figure 3. MagVigen™ Easy DNA Cleanup & Size Selection Kit (K61001-Easy) also works better for medium contanmination NGS DNA Library sample (adapter dimer ratio 68%). After clean-up, adapter dimer over library is 0.45% using MagVigen™ vs. 1.2% using AmPure XP.

Before DNA Clean-up	After DNA Clean-up

Figure 4. MagVigen™ Easy DNA Cleanup & Size Selection Kit (K61001-Easy) still works better for log contanmination NGS DNA Library sample (adapter dimer ratio 20%). After clean-up, adapter dimer undetectable using MagVigen™ vs. 0.6% using AmPure XP.

MagVigen™ DNA Size Selection magnetic nanoparticles and kits have been optimized to capture DNA with desired cutoff sizes: >50bp, >100bp, >150bp, >200bp, >300bp, >400bp, >500bp, >700bp.

Figure 5. MagVigen™ DNA Size Selection Capture DNA Fragments of Desired Size. A DNA ladder input was successfully captured for >500bp, >400bp, >300bp, >200bp.

Figure 6. shows the capture efficiency of the MagVigen™ beads for DNA fragments at a range of sizes in three different buffer conditions. A DNA ladder input was successfully captured for >150bp, >75bp, >35bp.

Figure 7. Non-hybridization based DNA capture with MagVigen™ beads can be used in a wide range of temperature conditions.

Figure 8. A use case. Customer requires 200bp cut-off and the same protocol as Company A XP beads. We can see MagVigen™ as an XP alternative: 1. At same ratio, with similar yield, MagVigen™ achieved higher fidelity. XP captured a lot 100bp. 2. From Ratio 0.8, we can see the backward trend (0.8 up) MagVigen™ has much higher sensitivity.

MagVigen™ DNA Size Range Selection Kits

MagVigen™ – DNA Cleanup & Size Selection Kits enable you to fine tune your buffer condition for DNA size range selection.

Cat# Kr61001-300/600, Kf61001-500 focus MagVigen™ provides fast and cost-effective solution to isolating DNA of desired sizes for high-quality library construction and Next-Gen Sequencing (NGS).

Figure 9. Left shows salmon genomic DNA was separated based on 2 different size range selections. 300bp<DNA<600bp can be isolated for NGS. Right shows a 500bp focused band was isolated from DNA ladder input using similar approach.

MagVigen™ DNA Easy Size Selection Kits

Figure 10. shows the sizing results by adjusting the ratios of beads volume vs. DNA sample volume. Themo Scientific GeneRuler 100 bp DNA ladder and 2% agarose gel were used.

MagVigen™ DNA Extended Long BP Size Selection Kits

Figure 11. NVIGEN’s MagVigen™ nanobeads (Cat# K61001-Extend) is used to size select DNA in extended DNA size range (500bp – 3000bp). 1000ng Fisher 10kb DNA ladder were size-specifically captured by adjusting volume ratio of beads to DNA volume. At 0.8 ratio, MagVigenbeads can capture DNA base pairs > 3000 bp with >80% yield, useful for longer bp DNA sequencing. Insert shows bioanalyzer result of the longer bp DNA cleanup & sizing at volume ratio of 0.8 of beads vs. DNA sample.

In Summary, MagVigen™ nanoparticles features:

• Volume Ratio or Streptavidin Based DNA Selection
• Long bp DNA Selection Available
• Precise Size Range Selection
• High Yield
• Easy Workflow
• Fast Magnetic Response

Learn more about the basics & development of DNA Size Selection and DNA cleanup,

Learn which scientists are citing this product series on what projects.

K61001 FAQ