DNA Sizing for NGS DNA Library Purification

Name	Application	Cat#	Price (1ml, USD)	Add to cart
MagVigen™ – Easy DNA Size Selection Kit	Magnetic nanobeas for ratio based DNA size 100~500bp  Product Sheet	K61001-Easy	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >700bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >700bp Capture Buffer, Elution Buffer  Product Sheet	K61001-700	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >500bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >500bp Capture Buffer, Elution Buffer  Product Sheet	K61001-500	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >400bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >400bp Capture Buffer, Elution Buffer  Product Sheet	K61001-400	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >300bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >300bp Capture Buffer, Elution Buffer  Product Sheet	K61001-300	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >200bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >200bp Capture Buffer, Elution Buffer  Product Sheet	K61001-200	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >150bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >150bp Capture Buffer, Elution Buffer  Product Sheet	K61001-150	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >100bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >100bp Capture Buffer, Elution Buffer  Product Sheet	K61001-100	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – >50bp DNA Selection Kit	Including MagVigen™ magnetic nanobeads, >50bp Capture Buffer, Elution Buffer  Product Sheet	K61001-50	1 ml $129.002 ml $199.005 ml $320.0060 ml $1,119.00450 ml $5,559.00
MagVigen™ – 500bp Focus, DNA Selection Kit	Including MagVigen™ magnetic nanobeads, DNA fragment size selection of 500bp Capture Buffers, Elution Buffer  Product Sheet	Kf61001-500 Focus	$339
MagVigen™ – 300/600 bp Range, DNA Select Kit	Including MagVigen™ magnetic nanobeads, DNA fragment size selection between 300 and 600 bp Capture Buffers, Elution Buffer  Product Sheet	Kr61001-300/600	$339

 MagVigen™ magnetic nanoparticles enable the purification of DNA products from salt and protein. They can be used for PCR product clean-up, NGS (next generation sequencing) library construction DNA size selection, terminator dye removal, and removal of other contaminants from assay samples, e.g. cell lysate. The purified DNA products can be further analyzed by gel electrophoresis, PCR quantification and DNA sequencing including NGS.

 

Example 1 MagVigen™ Easy DNA Size Selection Kit (K61001-Easy) outperforms conventional brand products for strongly contanminated NGS DNA Library sample (adapter dimer ratio 224%).

Before Purification	After Purification

Example 2 MagVigen™ Easy DNA Size Selection Kit (K61001-Easy) outperforms conventional brand products for significantly contanminated NGS DNA Library sample (adapter dimer ratio 186%).

Before Purification	After NVIGEN K61001-Easy Purification
Tested by Agilent 2100 12000 Assay	Tested by Agilent 2100 High Sensitivity Assay

Example 3 MagVigen™ Easy DNA Size Selection Kit (K61001-Easy) also works better for medium contanmination NGS DNA Library sample (adapter dimer ratio 68%).

Before Purification	After Purification

Example 4 MagVigen™ Easy DNA Size Selection Kit (K61001-Easy) still works better for log contanmination NGS DNA Library sample (adapter dimer ratio 20%).

Before Purification	After Purification

DNA Size Range Selection

MagVigen™ – DNA Size Selection Kits enable you to fine tune your buffer condition for DNA size range selection. The gel image below shows the capture efficiency of the MagVigen™ beads for DNA fragments at a range of sizes in three different buffer conditions. Try it for your sample prep today!

MagVigen™ DNA Size Selection magnetic nanoparticles have been optimized to capture DNA with desired cutoff sizes: >50bp, >100bp, >150bp, >200bp, >300bp, >400bp, >500bp, >700bp. The bottom and top figures shows a DNA ladder input was successfully captured for >500bp, >400bp, >300bp, >200bp, >150bp, >100bp, >50bp using each corresponding DNA size selection kits.

MagVigen™ DNA Size Selection Capture DNA Fragments of Desired Size

MagVigen™ DNA Size Range Selection Kits

Cat# Kr61001-300/600, Kf61001-500 focus MagVigen™ provides fast and cost-effective solution to isolating DNA of desired sizes for high-quality library construction and Next-Gen Sequencing (NGS). The figure on the bottom left shows salmon genomic DNA was separated based on 2 different size selections. 300bp<DNA<600bp can be isolated for NGS. A 500bp band was also isolated from DNA ladder input using similar approach.

MagVigen™ DNA Size Selection Ideal for DNA Library Construction

MagVigen™ – DNA Select allows simple and rapid isolation of DNA from a variety of crude sample materials (ex. blood sample, cell lysates, clinical specimens, and tissue samples). It enables efficient recovery of DNA and efficient removal of unwanted contaminants (ex. primers, unincorporated dNTPs, and salts)

Non-hybridization based DNA capture with MagVigen™ beads can be used in a wide range of temperature conditions.