MagVigen™ 150bp DNA Select Kit

Cat# K61001-150

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Product Description

MagVigen™ DNA Select nanoparticles are ideal for DNA purification. MagVigen™ DNA Select nanoparticles feature efficient recovery of double-stranded and single-stranded DNA. DNA products are captured by MagVigen™ DNA Select nanoparticles following a short incubation. The generated nanoparticle-oligo complex can be separated from the rest of the sample by magnet. The retained genomic material can be eluted from the nanoparticles using an elution buffer.

MagVigen™ DNA Select nanoparticles enable the purification of DNA products from salts. They can be used for PCR product clean-up, terminator dye removal, and removal of other contaminants from assay samples, e.g. cell lysate. The purified DNA products can be further analyzed by gel electrophoresis, PCR quantification and sequencing.

Product Contents

• MagVigen™ DNA Select nanoparticles (1ml)
• Capture Solutions > 150bp (2ml)
• Elution Buffer (2ml)

All materials should be stored at 4°C.

Shelf life: 6 months

Materials Needed

• 80% ethanol
• Magnetic rack, Cat# A20006

Protocol

Note:

The amount of nanoparticles needed for efficient DNA capture depends on the DNA concentration in the starting material.

In general, use 20μl of MagVigen™ DNA Select nanoparticles per 20μl of DNA sample.

Important: Always resuspend nanoparticles in 1.5X volume of Capture Solution for 1X volume of DNA sample.

Example: If the DNA sample is 20μl , then use 30μl of Capture Solution.

Size selection:

Capture Buffer >150bp: Recommended for DNA >=150bp

General DNA Capture Protocol

Bind DNA

1. Remove MagVigen™ DNA Select nanoparticles from storage and bring them to room temperature.
2. Vortex MagVigen™ DNA Select nanoparticles for 10 seconds before use.

Note: Make sure the beads are fully resuspended and well dispersed.

3. Transfer 20μl of MagVigen™ DNA Select nanoparticles and put into a clean 1.5ml micro-centrifuge tube.
4. Collect MagVigen™ DNA Select nanoparticles using magnetic rack and discard the supernatant.

Note: A clear precipitate containing dark brown colored nanoparticles should become visible on the side of the micro-centrifuge tube.

5. Resuspend the nanoparticles in 30μl of Capture Solution.
6. Add 20μl of DNA sample to the MagVigen™ DNA Select nanoparticles.
7. Vortex or pipette the reaction solution to mix thoroughly.

Note: It is ideal not to introduce bubbles during the capture reaction.

8. Incubate the MagVigen™ DNA Select nanoparticles-DNA reaction at room temperature for 30 minutes.
9. After incubation, use the magnet rack to separate the DNA- captured nanoparticles from the solution.
10. Carefully remove the supernatant with a pipette, make sure not to disturb the DNA-captured nanoparticle pellet.

Wash DNA

11. Keeping the magnet in place, rinse the DNA-captured nanoparticle pellet by adding 100μl of freshly prepared 80% ethanol. Let stand for 30 seconds-1 minute.

Note: Adjust the volume of ethanol as needed to sufficiently cover the DNA-captured nanoparticle pellet.

12. Remove and discard the ethanol.
13. Repeat steps 11-12, performing a total of two rinses.
14. Allow the sample to air dry at room temperature.

Note: Time will vary depending on the reaction volume. Try not to allow pellet to over-dry and crack. This could affect the recovery.

Elute DNA

15. Elute the captured DNA from the nanoparticles by adding 20μl of the Elution Buffer.

Note: The volume of the Elution Buffer can be adjusted as needed.

16. Gently pipette to mix well and incubate for 2 minutes at room temperature.

Note: It is ideal not to introduce bubbles during the elution reaction.

17. Spin down a few seconds to collect the solution.
18. Place tube on magnetic rack for 1 minute to separate the nanoparticles from the eluted DNA.
19. Transfer the supernatant containing the DNA products to a clean tube. Make sure not to disturb the pellet. The purified DNA is now ready to use for subsequent evaluation.

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