|Name||Application||Cat#||Price (1ml, USD)||Add to cart|
|MagVigen™ – Streptavidin DNA Capture||For sequence specific DNA Capture
|MagVigen™ – Streptavidin DNA Capture Kit||Include MagVigen™ – Streptavidin nanoparticles, wash buffer, blocking solution, capture solution.
|MagVigen™ – mRNA Select||For mRNA selection
|61004||$399||MagVigen™ – mRNA Select Kit||Include MagVigen™ – Poly T, wash buffer, and elution buffer
|MagVigen™ – DNA probe||Customized preparation of magnetic nanoparticle-DNA conjugates
|MagVigen™ – Oligo Conjugation Kit||To purify sequence specific DNA or mRNA
MagVigen™ magnetic nanoparticles are ideal for DNA purification with high magnetic response and superior biocompatibility, specifically tailored for the best applications in magnetic enrichment and purification, magnetofection, magnetic manipulation, magnetic sensing, and magnetic resonance imaging. They feature efficient recovery of double-stranded and single-stranded DNA. DNA is captured by MagVigen™ magnetic nanoparticles following a short incubation. The generated nanoparticle-oligo complex can be separated from the rest of the sample by a magnet. The retained genomic material can be retrieved from the magnetic nanoparticles using an elution buffer.
MagVigen™ – Streptavidin magnetic nanoparticle beads enable convenient capture of specific DNA sequence from biological samples, such as tissue lysate, whole blood and plasma samples. Captured DNA can be used in downstream applications such as NGS and clinical diagnosis. The figure below shows sequence specific DNA capture from human blood using MagVigen™ – Streptavidin magnetic nanoparticle beads and biotin-CD19-targeted DNA probes.
MagVigen™ magnetic nanoparticle beads have smaller nanoparticle size, hence higher surface area and higher binding capacity. Smaller quantity of magnetic beads solid material is needed to achieve same capture capacity of targets compared to other magnetic beads on market. As shown in the following figure, much less MagVigen™ – streptavidin magnetic nanoparticle beads are needed when compared to conventional brand name magnetic beads – streptavidin conjugates for sequence specific DNA capture. The smaller pellet size allows less buffer volume for elution, providing a higher concentration of target molecule solution for more sensitive downstream applications, such as NGS or DNA diagnosis. The smaller nanoparticle size, higher binding capacity, and unique surface chemistry prepared using NVIGEN’s proprietary technology make our MagVigen™ magnetic nanoparticle beads ideal for molecular and cellular purification and enrichment from complex biological samples. Shown in the following figure is for sequence specific DNA capture from fresh human whole blood samples using streptavidin conjugated magnetic beads and biotin-DNA probe. The conventional magnetic beads show severe aggregation. MagVigen™ magnetic beads enable a much cleaner magnetic capture result with much smaller pellet size and negligible aggregation for DNA extraction from whole blood samples.
Left side using brand name beads; right side using MagVigen™ – Streptavidin nanobeads.
Sequence specific DNA capture from whole blood samples.
- Capture specific DNA molecules directly from sample solutions
- Compatible with downstream applications (NGS, clinical diagnostics)
- Smaller beads -> higher binding capacity -> less elution volume -> Higher DNA concentration
- Better for probing protein-DNA interactions
MagVigen™ beads have high capture efficiency (80% – 100%). The capture efficiency is equivalent to AMPURE beads. In this experiment, 1µg of genomic DNA from OC1-LY8 cells and salmon sperm were used as starting materials.