Magnetic particles based procedures, also called as SPRI, for preparation and manipulation of high quality DNA are known to be rapid, low cost and procedurally identical for all DNA types and all scales of samples, from plasmids to single copy BAC clones.
The magnetic characteristics of the particles bring the SPRI technology a benefit to standardize all DNA manipulations and isolations and enable us to perform solid-phase biochemistry to encompass many steps in molecular biology.
Question: Given the same amount (mass) of particles, which one can carry more targets, bigger beads or smaller ones?
Assume the bigger beads are 10x in size, one bigger beads equals 1000 smaller ones with the same mass (spheric volume 4/3*PI*r3). The capacity to carry targets is proportional to the spherical surface area (4*PI*r2). That means one bigger bead has 100x surface area of 1 smaller bead. Sum the 1000 smaller ones, they actually total 10x surface area, i.e. 10x capacity.
And that’s one power of nanoparticles vs. conventional particles in µm.
The capability of beads comes from the functional groups assembled to beads, not directly from the beads themselves. Our MagVigen™, manufactured with the state-of-the-art nanotechnologies are coated and engineered at the molecular level to assemble a variety of functional groups, including proteins, nucleic acids, antibodies, peptides, ligands, drug molecules and signal particles.
Example: Fluorescent Magnetic Nanoparticles (FMN)
The picture shows a schematic illustration of creating multifunctional fluorescent magnetic nanoparticles. Starting from individual 8 nm superparamagnetic iron oxide (SPIO) cores (dark brown), a silanization shell is grown (yellow or tan), called siliceous coating, and then covalently linked to organic fluorophores (red), along with biofunctional molecules such as drugs or targeting molecules (green), called conjugating. The sizes of coated SPIO is 87nm and the product nanoparticles size 97nm.
The fluorescence of the particles permits direct imaging of the nanoparticles in a living subject through an intravital microscope, while the magnetic properties are suitable for magnetic targeting, which improves specific localization of nanoparticles when sufficient magnetic gradients are applied.
Injectable multifunctional fluorescent magnetic nanoparticles (FMNs). (A) Transmission electron micrograph showing FMNs with individual SPIO cores. Nanoparticles remain well dispersed . (B) Overlaid image showing the (red) fluorescence from systemically administered FMNs outlining tumor neovasculature and the (green) color showing EGFP-transfected human U87MG glioblastoma tumor cells. As shown, no aggregates of nanoparticles in the bloodstream were observed using optical microscopy and the FMN fluorescent signal smoothly outlines the tortuous tumor neovasculature, including the very thin capillaries that are only a few micrometers in diameter.
The development of NVIGEN nanoparticle platform is based on multifunctional fluorescent magnetic nanoparticles incorporating potentially biocompatible components. The SPIO core in these nanoparticles uses the same material as in the clinical MRI agent Feridex and the colloidal iron oxide for treating iron deficiency anemia. The SPIO cores are coated with a biocompatible siliceous shell, the material component similar to what’s used in daily calcium supplements. And the fluorescence is from covalently bonded organic fluorophores, and FDA-approved fluorophores are available.
Nanoparticles, cell imaging, in vivo imaging, nano drug in tumor neovasculature.
Advantages of NVIGEN’s nanoparticle reagents include:
- Optimal water dispersion
- Superior biocompatibility
- High affinity conjugations
- Increased stability
- Enhanced sensitivity
- Targeted delivery and specificity
- Payload applications
- Controllable pharmacokinetics
- Optical contrast enhancement and magnetic properties
- Size tunability
- Multiplex and high-throughput capability
Improve your essay and NGS DNA sample prep
We cordially invite you to try our state-of-the-art MagVigen™ DNA Sample prep kits (K61001) for your DNA purification and size selection needs.
Or interested in sequence specific DNA/RNA capture?
Check our our MagVigen™-Streptavidin products (K61002).